23 Jul Construction method of 3D epidermis model
Abstract
The invention belongs to the technical field of tissue engineering and particularly relates to a construction method of a 3D epidermis model. According to the method, a fibroblast treated by using mitomycin C (Mitomycin C, MC) is adopted as a trophoblast and inoculated with a keratinocyte, various factors and proteins secreted by the fibroblast are supplied to the keratinocyte for growth requirements, and meanwhile, low-concentration serum, EGF, insulin and CaCl2 are matched to construct the 3D epidermis model through two phases of submerged culture and gas-liquid surface culture. The constructed 3D epidermis model is highly consistent with a human epidermis structure, the production cost is reduced, and the economical, effective and efficient requirements required by large-scale production of the 3D epidermis model are met.
Year: 2017
Country: CN
Doc No: 107287150