23 Jul Construction method for human amniotic epithelial cells and silk fibroin support complexus
Abstract
The invention provides a method for constructing amniotic epithelial cells and silk fibroin support complexus in vitro, and the method comprises the following steps of: isolated culture of amniotic epithelial cells; purification of primary cells; identification of primary cells; amniotic cell inoculation on silk fibroin support for space cultivation; and result observation through a scanning electron microscope. According to the method, after human cesarean delivery, wasted amniotic membrane is taken as the material, the amniotic epithelial cells are obtained for cultivation in vitro, and cell purification is carried out under certain conditions, the purified cells and the silk fibroin support are used for constructing a tissue engineering material, thus building a good space growing environment for cells. The invention provides a new way for curing functional repair of all traumatic diseases of the central nervous system by using amniotic membrane cell transplantation in clinical application in the near future.
Year: 2013
Country: CN
Doc No: 103045535