23 Jul Conjugate of collagen material and growth factor as well as preparation method thereof
Abstract
The invention discloses a conjugate of a collagen material and a growth factor as well as a preparation method thereof. The method comprises the steps of: crosslinking the collagen material and 2-iminosulfane hydrochloride to obtain a conjugate I; crosslinking the growth factor and sulfosuccinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate to obtain a conjugate II; and mixing the substance I and the conjugate II for reaction to obtain the conjugate of the collagen material and the growth factor. Testes prove that by crosslinking the growth factor on the collagen material with a chemical crosslinking method, the invention ensures that the surface activity of the collagen material is effectively improved, the cellularization and vascularization degree of the collagen material can be obviously improved, and the collagen material can be more effectively applied to the medical fields of tissue engineering and the like. Therefore, the conjugate and the preparation method therefore in the invention have broad application prospect in the fields of tissue engineering and the like.
Year: 2011
Country: CN
Doc No: 101962409