23 Jul Collagen film with 3D micro-patterns, and preparation method and application thereof
Abstract
The invention belongs to the field of biomedical materials, and discloses a collagen film with 3D micro-patterns as well as a preparation method and application thereof. The collagen film with the 3Dmicro-patterns is prepared by the following steps: forming 3D micro-patterns on a silicon wafer by adopting a photolithography technology by using a mask with 3D micro-patterns; pouring PDMS prepolymers onto the silicon wafer so as to be subjected to cross-linking and curing, thereby obtaining a PDMS base with the 3D micro-patterns; dissolving collagen in an acidic solution, adding water, and carrying out uniform stirring so as to obtain a collagen solution; adding a cross-linking agent into the collagen solution so as to be subjected to cross-linking, thereby obtaining cross-linking reactionproducts; and then, removing bubbles from the cross-linking reaction products, pouring the bubble-removed cross-linking reaction products onto the PDMS base, carrying out air-drying so as to have a film formed, and carrying out stripping so as to obtain the collagen film with the 3D micro-patterns. The preparation technology of the collagen film with the 3D micro-patterns is simple in processes and relatively low in cost; and the prepared film combines advantages of collagen and patterned surface, thereby having good physicochemical properties and biocompatibility. The collagen film with the 3D micro-patterns can be used in corneal tissue engineering so as to induce corneal tissue regeneration; moreover, the collagen film with the 3D micro-patterns is capable of performing regulation on corneal cell behavior, thereby forming a structure similar to native tissues.
Year: 2019
Country: CN
Doc No: 109833515