23 Jul Cloning method of Nanjiang yellow goat GSK-3beta gene coding region complete sequence
Abstract
The invention belongs to the technical field of animal gene engineering, and specifically relates to a cloning method of Nanjiang yellow goat GSK-3beta gene coding region complete sequence. Coding region nucleotide sequence is represented by sequence table SEQ ID No:1. The invention also discloses a Nanjiang yellow goat GSK-3beta gene cloning method. The Nanjiang yellow goat GSK-3beta gene cloning method comprises following steps: step 1, total RNA of Nanjiang yellow goat liver tissue is extracted, and cDNA is obtained via reverse transcription; step 2, amplimer design and PCR reaction are carried out; step 3, amplification products are recovered and purified; and step 4, cloning is carried out. According to the cloning method of Nanjiang yellow goat GSK-3beta gene coding region complete sequence, Nanjiang yellow goat GSK-3beta gene complete coding region sequence is obtained, and theoretical foundation is provided for further research on function of Nanjiang yellow goat GSK-3beta gene.
Year: 2015
Country: CN
Doc No: 104694555