23 Jul Cloning and application of swine liver specificity expression gene TTR promoter
Abstract
The invention belongs to the technical field of animal gene engineering, in particular to cloning and activity analysis of a swine liver specificity expression gene TTR promoter with the length of 2205 bp. A 2205 bp-long regulatory sequence at the upstream of a translation initiation codon ATG of the swine liver specificity expression gene TTR promoter is cloned from a swine genome, and the nucleotide sequence is shown as the SEQ ID NO: 3; the result shows that the TTR promoter with the length of 2205 bp has independent activity and liver tissue specificity with the nucleotide sequence shown as the SEQ ID NO: 3. The invention further discloses a preparation method of the promoter fragment, a dual-luciferase activity detection system and the application of the RT-PCR method for the activity analysis of the promoter.
Year: 2013
Country: CN
Doc No: 103421793