23 Jul Clone and application of porcine skeletal muscle specific expression gene tnnc2 promoter
Abstract
The invention belongs to the technical field of animal genetic engineering, and particularly relates to separation identification and functional verification of promoter areas with different lengths of porcine skeletal muscle specific expression gene tnnc2. Five promoters with different lengths in the upstream of a porcine skeletal muscle specific expression gene tnnc2 are cloned from a porcine genome, and the nucleotide sequences are shown as SEQ ID NO: 1 to SEQ ID NO: 5; the result shows that tnnc2-pro fragment of 2313 bp and tnnc2-Q2 fragment of 1048bp have independent promoter activity and muscular tissue specificity, and the nucleotide sequences are shown as SEQ ID NO: 1 and SEQ ID NO: 3. The invention discloses a preparation method of five different deletion promoter fragments, a dual-luciferase activity detecting system and application to promoter activity analysis by a quantitative PCR method.
Year: 2013
Country: CN
Doc No: 103421789