23 Jul Cinnamomum kanahirai hay body embryo culture medium and tissue culture rapid propagation method
Abstract
The invention discloses a Cinnamonum kanehirae Hayata somatic embryo culture medium, which contains MS as a basic culture medium or MS salts and MT vitamin, and also contains growth regulators such as BA, Picloram, ABA and NAA, 170mg/L sodium dihydrogen phosphate monohydrate,30mg/L adenine sulfate, 0.5g/L caseinhydrolysate, 1.2 percent agaragar, and 3 percent saccharose, wherein three culture media for somatic embryogenesis, somatic embryo proliferation and embryo differentiation, and embryo rooting are prepared respectively. The tissue culture and rapid propagation method of Cinnamonum kanehirae Hayata by using the culture media comprises the following four steps: 1. the acquisition and disinfection of an explant; 2. the induction of a somatic embryo; 3. the somatic embryo proliferation and embryo differentiation; and 4. root induction and transplanting. The invention initially creates a complete set of method for Cinnamonum kanehirae Hayata somatic embryos to reproduce seedlings, saves the endangered species resource, lays a foundation for further exploring the gene engineering of somatic embryos, broadening explant materials and so on, and provides adequate wood for the mass production of Antrodia camphorata with extremely high economic value.
Year: 2009
Country: CN
Doc No: 101429489