23 Jul Chitosan modified intelligent tissue engineering scaffold material and preparation method thereof
Abstract
The invention discloses a chitosan modified intelligent tissue engineering scaffold material and a preparation method thereof. The invention belongs to the field of the functional macromolecular material and particularly relates to the intelligent tissue engineering scaffold material and the preparation method thereof. The temperature response range of the scaffold material is 31-33 DEG C. the preparation method of the scaffold material comprises the following steps: adopting the ultraviolet curing method to dissolve temperature sensitive substances N-isopropylacrylamide (NIPAAm) and acrylic acid (AAc) in dimethyl sulfoxide (DMSO) according to a certain ratio, using anthraquinone-2-sulfonic acid (AQS) as a photosensitizer to graft on the surface of a polystyrene cell culture plate, and then using N-hydroxysulfosuccinimide sodium salt (sulfo-NHS) and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC.HCl) as an activator and a condensing agent to fix chitosan (Cs) with good biocompatibility on the surface of the polystyrene cell culture plate and prepare the chitosan modified intelligent tissue engineering scaffold material. The preparation method disclosed by the invention has a simple process, high maneuverability, low investment cost, a wide application range and safety and is easy to be popularized and used in industrial production.
Year: 2011
Country: CN
Doc No: 102250372