23 Jul Cell three-dimensional culture method for tissue engineering
Abstract
The present invention discloses one kind of 3D cell culture method for tissue engineering, and belongs to the field of tissue engineering technology. Into various kinds of biological rack material, thrombin in working concentration of 100-500 IU/ml and calcium chloride and other catalyst in working concentration of 20-60 mmol are added. Various kinds of cell are suspended in the culture medium with fibrinogen in working concentration of 10-50 mg/ml and XIII factor in working concentration of 10-50 U/ml, and inoculated in various kinds of rack material homogeneously based on hematopexis principle, so as to obtain 3D cell-rack material-fibrin gel culturing system. By means of the said method, excellent 3D cultured cell may be obtained for constituting various kinds of engineering tissue.
Year: 2007
Country: CN
Doc No: 1928077