23 Jul Cell culture device capable of loading shear force stimulation and electrical stimulation jointly in parallel direction
Abstract
The invention discloses a cell culture device capable of loading shear force stimulation and electrical stimulation jointly in the parallel direction. The cell culture device comprises a cell culture device, a peristaltic pump, a signal generator, an upstream liquid storage cylinder and a downstream liquid storage cylinder, wherein the cell culture device consists of a tank body, a flat plate, a silica gel gasket, a cell culture substrate, a cover and a bolt; the peristaltic pump, the upstream liquid storage cylinder, the downstream liquid storage cylinder and the like are connected by a silicone tube to form a culture solution perfusion system; a circular cell culture solution passes through the cell culture device, so that the flowing shear force stimulation is loaded to cells; and the signal generator is connected with a metal electrode on the cover of the tank body of the cell culture device, so that the uniform electrical stimulation of which the direction is parallel to that of shear force is loaded to the cells. By the cell culture device, the defect that the simulation of in-vivo cells in microenvironment in which the shear force stimulation and the electrical stimulation exist simultaneously cannot be performed by the conventional method is overcome, cell culture environment in which the shear force stimulation and the electrical stimulation are loaded jointly in the parallel direction is established, and the development of relevant tissue engineering reactors is expanded.
Year: 2012
Country: CN
Doc No: 102559492