23 Jul Cell co-culture method for improving proliferation activity of somatic cells
Abstract
The invention discloses a cell co-culture method for improving the proliferation activity of somatic cells. The cell co-culture method comprises the following steps of: transfecting suicide genes into stem cells; co-culturing the somatic cells and the stem cells transfected with the suicide gene; and after the somatic cells are proliferated, starting the suicide genes in the stem cells to remove the stem cells. The stem cells carrying the suicide genes and the somatic cells are co-cultured, the proliferation activity of the somatic cells is improved at the presence of the stem cells, a small number of somatic cells can quickly grow in short time, and sufficient somatic cells are obtained; and when the number of the somatic cells is large enough, the suicide genes carried in the stem cells are started to induce the apoptosis of the stem cells, and the pure somatic cells with high proliferation activity are obtained, and do not have tumorigenicity after transplantation, so that the method is great breakthrough for cell engineering and tissue engineering, and is a new way for next-generation clinical application. The method has the advantages of reliable principle, simple steps and stable results, and is easily applied to experimental study and clinical treatment in the field of bioengineering.
Year: 2012
Country: CN
Doc No: 102559578