23 Jul Cell co-culture method capable of realizing in-vitro preparation of liver buds and liver buds
Abstract
The invention relates to a cell co-culture method capable of realizing in-vitro preparation of liver buds. The cell co-culture method comprises the following steps: (1) respectively digesting hepatocyte-like cells, endothelial progenitor cells and mesenchymal stem cells, resuspending the digested cells in a culture medium, and carrying out mixing according to the number ratio of the hepatocyte-like cells to the endothelial progenitor cells to the mesenchymal stem cells being 1: (0.5-1.5) to (0.3-1), thus obtaining a mixed cell suspension, wherein the hepatocyte-like cells are obtained by carrying out induced directional differentiation on induced pluripotent stem cells; and (2) uniformly planting the mixed cell suspension in a culture dish, and carrying out culturing for 3-7 days till the liver buds are formed. According to the cell co-culture method, the in-vivo living environment of the cells is simulated as far as possible, the culturing system is optimized, the defects and deficiency existing in the conventional cell culture are broken through, finally, a tiny three-dimensional structure is generated through cells, therefore, a novel research field is developed for the tissue engineering field, and a novel thought and a novel method are provided for the regenerative medicine research.
Year: 2017
Country: CN
Doc No: 106367380