23 Jul Cell assembling small-intestinal submucosa bionic composite engineering bone and preparation method thereof
Abstract
The invention discloses a cell assembling small-intestinal submucosa bionic composite engineering bone; the cell assembling small-intestinal submucosa bionic composite engineering bone has higher mechanical strength; the elasticity modulus is 350 MPa and 100 MPa respectively in dry and wet states; better tissue support can be provided in an implant in the initial stage; the tissue engineering bone is very close to a mineralized structure and distribution in a natural bone; the tissue engineering bone has a micro-structure highly similar to the natural bone; after being implanted, the tissue engineering bone is beneficial to well identifying and accelerating osteanagenesis; a preparation method of the bionic composite engineering bone disclosed by the invention is a novel combination inducing osteoblast differentiating and mineralizing method; osteoblast on SIS (Styrene Isoprene Styrene) is stimulated through calcium ion and icariin combined culture solution; a lot of in-situ mineralizing and osteogenic differentiation on the SIS material are carried out; simultaneously, inhibition of calcium ion to important matrix protein OCN in the later differentiation period can be avoided; the bone conductivity of the engineering bone is enhanced; a lot of hydroxyapatite crystallization and osteoblastic-like cell epimatrix deposition on the SIS material are finally realized; and furthermore, compared with SIS self, the mechanical strength is greatly increased.
Year: 2015
Country: CN
Doc No: 104874024