23 Jul Carrier-free cell sheet and preparation method and application thereof
Abstract
The invention belongs to the field of tissue engineering, and particularly relates to a carrier-free cell sheet and a preparation method and application thereof. The preparation method of the carrier-free cell sheet comprises the steps of transfecting the suicide gene into a supporting cell; planting the supporting cell with the transfected suicide gene at the bottom of a culture container; planting target cells on the supporting cell; after realizing good fusion between the target cells and establishing intercellular connection, starting the suicide gene in the supporting cell for apoptosis to obtain a carrier-free cell sheet containing pure target cells. By adopting the method provided by the invention, a carrier-free cell sheet with good biological characteristics can be obtained, which is a new breakthrough in establishing a carrier-free cell sheet in the field of tissue engineering; meanwhile, the product also provides a feasible scheme for clinical disease treatment. The principle is scientific and reliable, and the process is simple and flexible.
Year: 2014
Country: CN
Doc No: 103642749