23 Jul Blood vessel vitrifying storage liquid and blood vessel storage method
Abstract
The present invention relates to blood vessel storing liquid for fast vitrifying and cryogenically storing body blood vessel, artificial blood vessel and tissue engineering blood vessel and the corresponding storage method. The blood vessel vitrifying and storing liquid is compounded through diluting polysaccharose and mycose with M199 diluent to obtain mixed solution of 20-35 % and 15-25 % concentration separately; diluting vinyl glycol and vinyl alcohol with the mixed solution to mixed liquid of 20 % concentration; and adding azone as high penetrant in 0.5-1 % to form the blood vessel vitrifying and storing liquid, into which, 0.001-0.005 % of cationic surfactant may be also added. Blood vessel is thrown into the vitrifying and storing liquid inside some plastic bag at room temperature, and the sealed bag is thrown into liquid nitrogen for cryogenical storing. The cryogenically stored blood vessel may be defrosted via taking out the bag from liquid nitrogen and soaking in water bath at 22-25 deg.c for 15 sec.
Year: 2004
Country: CN
Doc No: 1491538