23 Jul Biomimetic porous microsphere tissue engineering scaffold and manufacturing method thereof
Abstract
The invention discloses a biomimetic porous microsphere tissue engineering scaffold and a manufacturing method thereof. The scaffold is a microsphere manufactured by imitating natural extracellular matrix; the diameter ranges from 100 micrometers to 500 micrometers; cell holes and material exchange holes are distributed on the surface of the scaffold; three-dimensional network structures which are communicated with each other are arranged in the scaffold. When in use, a certain quantity of the scaffolds form various scaffolds which can be seen by the naked eyes by using a tailor made die or atissue engineering reactor. The scaffold comprises natural biological materials of collagen and chitosan, and the manufacturing method of the scaffold comprises the following steps of: (1) mixing 8 to 10 percent of collagen acetic acid dispersion liquid and chitosan acetic acid dispersion liquid in certain proportion; (2) mixing mixed liquid obtained in the step (1) and liquid paraffin containing6 to 8 percent of Span-85; (3) adding mixed liquid obtained in the step (2) to the liquid paraffin in volume ratio of 1 to 5, severely stirring and crosslinking with glutaraldehyde to obtain a crude product of the tissue engineering scaffold; and (4) respectively washing the crude product obtained in the step (3) with distilled water, isopropyl alcohol, ether and absolute ethyl alcohol and screening to obtain a finished product of the tissue engineering scaffold. The invention can be used as a tissue engineering scaffold, inhibit the tumor-induced trend in the process of stem cell growing development and promote the regeneration of blood vessels and nerve, and the rate of degradation and absorption is adapted to the formation of tissue organs. In addition, the invention can also be used for injectable tissue engineering scaffolds, great proliferation of seed cells used for tissue engineering or vectors for stem cell transfer for treatment.
Year: 2010
Country: CN
Doc No: 101766837