23 Jul Biological sample preparation method applicable to ultra-thin section and fluorescence imaging
Abstract
The invention relates to a biological sample preparation method applicable to ultra-thin section and fluorescence imaging and belongs to the technical field of biological engineering. The method comprises the following steps of: fixing biological tissue which is marked by fluorescent protein by using 4 percent paraformaldehyde; after a sample is rinsed in a phosphate buffer solution (PBS) with concentration of 0.01 MOL/L, performing gradient dehydration by using an ethanol solution, wherein dehydration concentration does not exceed 95 percent; and permeating and embedding the sample by using improved glycidyl methacrylate (GMA). An embedded biological sample which is prepared by the method has hardness for continuous ultra-thin section and can keep a fluorescence signal. The method is simple, low in cost and suitable for popularization in common laboratories.
Year: 2012
Country: CN
Doc No: 102620966