23 Jul Biological fluid film of recombined human keratinized cell growth factor-2 and its preparing process
Abstract
The present invention is recombinant human keratinized cell growth factor-2 (rhKGF-2) biofluid membrane for treating epithelium tissue damage and its preparation process. The rhKGF-2 biofluid membrane includes purified rhKGF-2 liquid and biofluid dressing comprising chitosan, gelatin, glycerin and PVP. The purified rhKGF-2 liquid is prepared through connecting KGF-2 gene onto the expression plasmid pLY4 to constitute expression vector transforming colibacillus and clone engineering bacillus; culturing engineering bacillus and raising temperature to induce expressing rhKGF-2; and separating and purifying the expressed rhKGF-2. The biofluid dressing is prepared is prepared with chitosan as main matrix, and gelatin, glycerin and PVP as supplementary material in optimized compounding proportion.
Year: 2005
Country: CN
Doc No: 1654071