23 Jul Artificial intervertebral disc complex tissue and preparation method thereof
Abstract
The invention relates to the field of tissue engineering, and discloses an artificial intervertebral disc complex tissue and a preparation method thereof. The artificial intervertebral disc complex tissue comprises nucleus pulposus and fibrous rings, wherein the fibrous rings consist of bone matrix rings of demineralization cells; the nucleus pulposus is formed by growing nucleus pulposus cells and fibrous ring cells on a nucleus pulposus-fibrous ring complex scaffold consisting of collagen II, hyaluronic acid and chondroitin sulfate. The shape and the size of the artificial intervertebral disc are similar to those of the natural intervertebral disc, the outer fibrous ring tissue is dense, and the inner nucleus pulposus is of semitransparent gel. The detection results of biochemical components show that the contents of DNA, protein polysaccharide and hydroxyproline of the artificial intervertebral disc complex tissue are remarkably increased along with the time and similar to the content of the natural intervertebral disc at the 12th week. The gene expression analysis result of collagen also shows that the complex intervertebral disc of the tissue engineering is similar to the natural intervertebral disc, and shows that the artificial intervertebral disc complex tissue has good biological function and wide application prospect.
Year: 2011
Country: CN
Doc No: 101954123