23 Jul Application of Myf6 promoter in driving expression of MyoD gene in muscular tissue
Abstract
The invention belongs to the field of animal genetic engineering, and in particularly relates to an application of a promoter region of a specific expression gene Myf6 of skeletal muscle of pigs in level verification of a model animal transgenic mouse living body. According to the invention, a constructed eukaryotic expression vector pSV40-Myf6-MyoDEGFP-N1 is subjected to linear treatment, and a transgenic mouse is prepared through micro-injection; MyoD gene expression conditions in different tissues of the positive transgenic mouse are verified, a tissue expression profile is established, and the horizontal expression conditions of MyoD in skeletal muscle of the transgenic mouse and wild type mouse in mRNA and protein are verified, the result shows that Myf6 gene promoter can specifically up-regulate the overexpression of the MyoD gene in the skeletal muscle; and the growth condition of muscle fiber is observed through a muscular tissue slice, the result shows that the area and the diameter of the muscle fiber in the muscular tissue slice of the transgenic mouse are significantly higher than those of the wild type mouse, and the density of the muscle fiber in unit area is obviously lower than that of the wild type mouse.
Year: 2015
Country: CN
Doc No: 105039340