23 Jul Alginic acid-hyaluronic acid in situ tissue engineering cell scaffold and its preparation method
Abstract
The invention provides an alginic acid-hyaluronic acid in situ tissue engineering cell scaffold and its preparation method. The preparation method comprises the following steps: alginic acid or its derivative and hyaluronic acid or its derivative are respectively made into solutions by the use of normal saline or physiological buffer; any one of the above solutions is mixed with cells which need to be loaded and then is mixed with the other remaining solution; and balancing lasts for a period of time at room temperature so as to obtain the alginic acid-hyaluronic acid in situ tissue engineering cell scaffold. The prepared alginic acid-hyaluronic acid in situ tissue engineering cell scaffold effectively prolongs degradation time of alginic acid and hyaluronic acid in vivo. By appropriate intramolecular crosslinking, mechanical properties and tissue filling/supporting performance of the scaffold are raised greatly. The scaffold has good security and validity. The preparation method of the alginic acid-hyaluronic acid in situ tissue engineering cell scaffold does not involve any organic reagent; the reaction system is simple and mild; and clinic application security is good.
Year: 2014
Country: CN
Doc No: 103550830