23 Jul Alginate/nano-clay composite microcarrier and preparing method and device
Abstract
The invention belongs to the technical field of tissue engineering biological scaffold materials and manufacturing thereof, and discloses an alginate/nano-clay composite microcarrier and a preparing method and device. The method comprises the steps of firstly, preparing a sodium alginate solution and nano-clay sol separately, and obtaining sodium alginate/nano-clay nano-composite hydrogel after the sodium alginate solution and the nano-clay sol are uniformly mixed. Furthermore, a stem cell suspension is mixed in the hydrogel, and besides, bioactive factors or drug ingredients can also be added. Then, the electrostatic drop method microcarrier preparing device which is mainly composed of a high-voltage power supply and a micro-injection pump is adopted, an open glass container containing acalcium chloride solution is used as a receiving device of the microcarrier, and proper injection speed and voltage are set. Compared with a pure alginate microcarrier, the prepared alginate/nano-claycomposite microcarrier has better mechanical performance, has the sustained drug release capability with which the alginate microcarrier is not endowed, and has an excellent application prospect in the field of stem cell treatment and tissue engineering.
Year: 2019
Country: CN
Doc No: 109646713