23 Jul A saussurea involucrata cell phenylalanine ammonia lyase gene SiPAL, a coding product thereof and applications of the gene
Abstract
A saussurea involucrata cell phenylalanine ammonia lyase gene SiPAL, a coding product thereof and applications of the gene are provided. The SiPAL gene is obtained by sequencing a transcriptome of saussurea involucrata cells and by analyzing and screening the sequencing results. The cDNA length of the gene is 2405 bp. The open reading frame is between 120 bp and 2264 bp. The nucleotide sequence of the gene is shown as SEQ ID NO:1. The phenylalanine ammonia lyase can be prepared by utilization of an SiPAL recombinant expression vector obtained by connecting the gene and a plant expression vector, and can be used for preparation of cinnamic acid by adopting L-phenylalanine as a substrate. By utilization of the gene and the coding product, the content of syringin that is a natural medicinal component can be increased by a genetic engineering technique especially in the field of cell and/or tissue culture of saussurea involucrate and plants of adjacent categories.
Year: 2015
Country: CN
Doc No: 104894150