23 Jul Molcular method for removing specific tissue of transgenic plant or target gene in organ by utilizing location recombination system
Abstract
The present invention provides site-specific recombination system induction controllable genetic manipulation of for deleting selected useless or detrimental gene from contemporary or progeny specific tissue or organ of transgenic plant. In the upstream and downstream of the set DNA sequence the site which can be identified by specific recombinase is set. It utilizes the programs of pollination or transformation to make the recombinase gene come into the controlled genetic engineering body, or utilizes the method of inducing gene expression to activate the recombinase gene of original specificity promote controlled by organ, and the recombinase can delete selected DNA sequience in specific time and specific position. Said invented method also possesses important application value in the gene function analysis.
Year: 2001
Country: CN
Doc No: 1292416