23 Jul Preparation and application of cell-eliminating pig corium substrate without cytotoxicity
Abstract
The preparation process of cell-eliminating pig corium substrate includes sustained vibration of the corium substrate in 0.5 mol/L NaOH solution compounded with thrice distilled water, rinsing with thrice distilled water and balanced phosphate salt solution separately, freeze drying, packing and sterilizing. The cell-eliminating pig corium substrate is used as the in vitro corium culturing rack in composite skin preparing tissue engineering process, and the preparation process includes coating the corium substrate with acetic acid dissolved type-I ox collagen, regulating pH to 7.0-7.4, soaking subsequently in balanced phosphate salt solution and no serum culture medium, inoculating passage epidermal cell, and no serum culture inside a CO2 hatcher. The tissue engineering composite skin is used as human skin substitute.
Year: 2005
Country: CN
Doc No: 1587390