23 Jul Bioreactor for vascellum tissue engineering
Abstract
The invention discloses a bioreactor in vascular tissue engineering, which comprises a hemodynamics simulating device, a 3D bracket and culture chambers. The hemodynamics simulating device is a pulsating flow control device; the culture chambers are serially connected with each other in the pulsating flow control device and include hermetically matched rotary devices and a sealed cavity; the rotary device includes a rotary rod having the 3D bracket fixed thereon and a drive device for rotating the rotary rod; and the rotary rod is located in the sealed cavity. The bioreactor can provide a pulsating flow simulating environment of human body facilitating growth, proliferation and differentiation of cells by adopting the pulsating flow control device for providing the dynamic effect simulating human heart. In the culture chambers, cells are inoculated in the internally-rotating 3D bracket, and secondary cell inoculation can be further executed to achieve convenient taking and dropping of cells, so that the cells can uniformly adhered on the 3D bracket, thus obviating the contamination of the reactor connected with the 3D bracket, on which the cells are inoculated through rotation.
Year: 2008
Country: CN
Doc No: 101294131