23 Jul Jellyfish genome DNA extracting method
Abstract
The invention relates to a jellyfish genome DNA extracting method, which belongs to the gene engineering technology. The method comprises the steps that the jellyfish is snipped into tissue without obvious particle by an Operating scissor, and CTAB buffer and protease K are added and digested for 24 hours to 26 hours at the temperature of 55 DEG C to 56 DEG C until the solution is clarified; the jellyfish also can be put into alcohol of 95 percent and dewatered, then buffer, SDS and the protease K are added, and after being sealed, the jellyfish is digested for 0.8 hours to 1.0 hour at the temperature of 55 DEG C to 56 DEG C; phenom, chloroform and isoamyl alcohol are mixed, centrifuged, and the suspernatant is absorbed; the mixed solution of chloroform and isoamyl alcohol is mixed evenly, centrifuged, and the suspernatant is absorbed; isopropyl alcohol is added into the supernatant fluid and deposited for 10 minutes to 15 minutes and taken out after deposition, the supernatant fluid is centrifuged, the liquor is discarded, and ethanol is used for being centrifugally washed, and finally the excess liquor is poured and naturally dried at the room temperature, thus the jellyfish genome DNA can be obtained. Through adopting the method, the jellyfish genome DNA with high quality can be extracted, therefore, the method has the advantages of simplicity, economical efficiency and easy operation, thereby being suitable for ordinary bilogical laboratories.
Year: 2009
Country: CN
Doc No: 101343631