23 Jul Activin A-containing serum-free culture solution for industrially producing bioreactor tissue engineering active artificial skin and preparation method thereof
Abstract
The invention belongs to the field of biomedicine, relating to a culture solution for producing bioreactor cell tissue engineering active artificial skins. The invention provides the culture solution for producing the tissue engineering active artificial skins and the specific preparation method thereof. The culture solution comprises the following components: 3-30ng/mL of cell growth factors, 2-30 mu g/mL of insulin, 0.2-0.6 mu g/mL of hydrocortisone, 3-30 mu g/mL of hypophysis extractive, 0.5-5.5 mmol/L of calcium ion, 3-30 mu g/mL of transferring, 20-60 U/mL of penicillin, 20-60 mg/mL of streptomycin and 0.1-0.9 ng/mL of activin A. The culture solution provided by the invention is suitable for preparation, has stable performance and long quality guarantee period and is convenient for large-scale synthesis of products and mass production.
Year: 2010
Country: CN
Doc No: 101857853