23 Jul Method for separating and purifying human amniotic fluid-derived mesenchymal stemcells
Abstract
The invention provides a method for separating and purifying human amniotic fluid-derived mesenchymal stemcells from discarded supernatant of prenatal diagnosis amniotic fluid culture. The cells are fibroblast-like amniotic fluid-derived mesenchymal stemcells obtained by extremely low-density cultivation in a specific culture medium, have multi-directional differentiation capability, have high amplification capability in comparison with adult cells and can maintain the original characteristics in 15 generations. The method has the main advantages that: (1) raw materials of the method are readily available and have no ethical tissues and an extremely high use value; (2) the cells obtained by using the method have high amplification capability, can maintain the characteristics in 15 generations and have the multi-directional differentiation capability; (3) the cell producing method of the invention is simple and high in efficiency, flow type and magnetic bead separation and purification processes and other separation and purification processes are not required, and the cost for obtaining the high-purity human amniotic fluid-derived mesenchymal stemcells is greatly reduced; and (4) the cells of the invention are applicable to tissue engineering, cell treatment and medicament screening and are suitable to be used as seeded cells of the tissue engineering.
Year: 2011
Country: CN
Doc No: 101948798