23 Jul Method for guiding fixed-point cell growth by preparing chemical micro-patterns on surfaces of various materials
Abstract
The invention discloses a method for guiding fixed-point cell growth by preparing chemical micro-patterns on the surfaces of various materials. The preparation process comprises the following steps of: 1) hydroxylating the surfaces of a glass sheet, a quartz sheet, a silicon sheet, a transition metal oxide and the like and coating dopamine on the surface of polymer or other inorganic materials; 2) obtaining dense anti-protein adsorption films on the pretreated surfaces by molecular self-assembly or an atom transfer radical polymerization method, wherein the dense anti-protein adsorption films are polyethylene glycol films or polyethylene glycol methacrylate films; and 3) covering mask plates on the anti-protein adsorption films, performing ultraviolet irradiation and adding fibronectin to obtain protein micro-patterns; and inoculating cells to obtain the cell patterns. The method has the advantages of simple operation, low cost, mild preparation conditions and no need of super clean experimental conditions. By adopting the method, stable and high-selectivity cell micro-patterns can be obtained on the surfaces of various materials; and the method has a broad application prospect in the fields of basic researches on cell biology, tissue engineering, drug screening and cell-based biosensors.
Year: 2011
Country: CN
Doc No: 101955595