23 Jul Skin substitute for automatically capturing endothelial progenitor cells and promoting vascularization and construction method thereof
Abstract
The invention relates to the technical field of tissue engineering and medical wound healing. The conventional skin substitute has low vascularization speed and low survival rate, and cannot be clinically popularized and applied after being grafted at present. The invention constructs a novel skin substitute capable of automatically capturing endothelial progenitor cells in peripheral blood and accelerating vascularization. A stroma cell derived factor 9HRE-CMV gene regulated by a hypoxic response element is used to transfect fibroblasts; epidermal cells and transgenic fibroblasts are implanted on both sides of a dermal scaffold respectively to form the sandwich skin substitute; and the skin substitute duly and moderately expresses and secretees SDF-1 alpha after being grafted, and endothelial progenitor cells (EPC) in the mediated peripheral blood are mediated to a skin substitute grafting place so as to promote the vascularization of the skin substitute and improve the survival rate after grafting. The skin substitute can obviously improve the chemotactic induction to the EPC and accelerates the vascularization of the skin substitute. The average survival rate of the skin substitute is 95 percent, while the average survival rate of the conventional skin substitute not containing the transgenic fibroblasts is 72 percent.
Year: 2011
Country: CN
Doc No: 101985052