23 Jul Method for preparing protein-loaded tissue engineering fiber support
Abstract
The invention relates to a method for preparing a protein-loaded tissue engineering fiber support in the technical field of medicaments, which comprises the following steps of: dissolving protein in macromolecular polysaccharide aqueous solution and using as an inner water phase; dissolving sustained-release macromolecules in an organic solvent, adding a suspending aid for dispersing, and using as an outer oil phase; adding the inner oil phase into the outer water phase dropwise, magnetically stirring or homogenizing to form emulsion, adding the emulsion into an injector, and performing electrostatic spinning by using a micro-injection pump and a receiver at room temperature under the condition of applying high-voltage static electricity to form a fiber membrane, or respectively putting the inner water phase and the outer oil phase into a capillary tube subjected to coaxial cospinning, and performing the electrostatic spinning by using the micro-injection pump and the receiver at room temperature under the condition of applying the high-voltage static electricity on inner and outer layers simultaneously; and standing and airing at room temperature to obtain tissue engineering fibers. The tissue engineering fiber support comprises the following components in percentage by weight: 0.5 to 20 percent of protein or polypeptide medicaments, 2 to 20 percent of macromolecular polysaccharides, 0 to 15 percent of suspending aid and 60 to 99 percent of sustained-release macromolecules. By the method, release curves of the protein are improved, the stability in the processes of preparation, storage and release is improved, and the medicament loading capacity of the fibers is increased.
Year: 2011
Country: CN
Doc No: 102188755