23 Jul Preparation method of three-dimensional bone-like tissue used for study of microgravity effect stimulation
Abstract
A preparation method of a three-dimensional bone-like tissue used for the study of microgravity effect stimulation relates to a preparation method of a three-dimensional bone-like tissue. The invention is to solve the problem that two-dimensional osteoblast models used for the study of microgravity effect stimulation at present can not truly represent the process of bone loss during spaceflight, and can not truly represent intercellular biological characteristics of in-vivo tissues. The method comprises the following steps of: 1, the mixing of a PBS buffer or normal saline, sodium alginate, gelatin and hydroxyapatite; 2, the induction of osteoblasts; 3, the induction of osteoclasts; 4, the preparation of microspheres for enwrapping the mixed cells; 5, the formation of the three-dimensional bone-like tissue. The invention forms a three-dimensional stent by sodium alginate, and embeds osteoblasts and osteoclasts together in a sodium alginate microsphere; the three-dimensional culture can simulate the growth condition of cells in a physiological state better, which solves the deficiency of existing two-dimensional osteoblast models. The invention is applicable to research fields of space biology, aerospace medicine and tissue engineering.
Year: 2011
Country: CN
Doc No: 102206608