23 Jul Crosslinking-technology-based bionic bone interface, and construction method and application thereof
Abstract
The invention relates to a crosslinking-technology-based bionic bone interface, and a construction method and application thereof. In the invention, biology signal-recombinant fibronectin/cadherin and a calcium phosphate ceramic micropore interface are subjected to covalent coupling. The invention aims to promote the high-efficiency adhesion and targeted differentiation of cells on the interface and belongs to the field of interface tissue engineering. The signal protein is recombinant protein of fibronectin type III (FNIII)7-10 carrying Pro-His-Ser-Arg-Asn (PHSRN) and Arg-Gly-Asp (RGD) motifs and cadherin functional domain EC1-2. crosslinking molecules are same bifunctional crosslinking agent ditertiary butyl peroxide (DTBP), and protein and materials can be bridged through amino acetylation. Plantation density and mineralization capacity of seed cells on the interface are improved through isophil and heterophilic adhesion of the signal protein and integrin subtype mediated osteoblasts induction. The interface characteristics are identified in the aspects of appearance topology, surface chemistry, cytocompatibility and the like during implementation. The crosslinking-technology-based bionic bone interface is suitable for preparation of alternative materials of bioactivity tissue engineering of compound cells.
Year: 2012
Country: CN
Doc No: 102430153