23 Jul Tissue-engineered atrioventricular node and construction method and application thereof
Abstract
The invention relates to the technical field of biomedical engineering. At present, the research about the construction of a tissue-engineered atrioventricular node is not reported in China and foreign countries. The invention provides a method for constructing the tissue-engineered atrioventricular node by using cardiac pacing cells and conduction cells which are obtained by inducing collagen sponge and bone marrow mesenchymal stem cells. The bone marrow mesenchymal stem cells are induced into the pacing cells through co-culture with sinoatrial node cells, and are induced into the cardiac pacing cells by a method for co-culture with auricular appendage muscle cells; and the two kinds of cells are used as seed cells, the collagen sponge is taken as a bracket, and the tissue-engineered atrioventricular node with a pacing characteristic and an electrocardio-conduction characteristic is constructed in vitro. Through animal experiments, the successfully constructed tissue-engineered atrioventricular node is transplanted to treat atrioventricular conduction block, and a good treatment effect is achieved. The tissue-engineered atrioventricular node which has the electrocardio-conduction characteristic and can be transplanted for treatment is obtained, and hope is brought to treatment of arrhythmia caused by heart atrioventricular conduction block.
Year: 2012
Country: CN
Doc No: 102488923