23 Jul Lattice-type three-dimensional cell culture support and its manufacturing method and use method
Abstract
The invention relates to a lattice-type three-dimensional cell culture support and its manufacturing method and use method. The lattice-type three-dimensional cell culture support comprises a quartz optical template with micropores. The quartz optical template with the micropores is made of a quartz optical template with films. The micropores form an inclined column-type micropore array. The manufacturing method comprises the following steps of preparing the quartz optical template with the films, then preparing the inclined column-type micropore array, and removing the film of a polymer material. The use method of the lattice-type three-dimensional cell culture support comprises high temperature disinfection of the lattice-type three-dimensional cell culture support, surface modification, cell implantation and culture, cell fixation, cell permeabilization and enclosing, cell incubation and cell cleaning. The manufacturing method provided by the invention can adjust aperture sizes, distribution and crystal lattice layer number of the lattice-type three-dimensional cell culture support, can effectively improve scientific and complete experimental data for the tissue engineering research, can improve cell culture efficiency and can reduce the frequency of animal experiments. The lattice-type three-dimensional cell culture support is suitable for large-scale production, has a low cost and can satisfy wide requirements of regenerative medicine.
Year: 2012
Country: CN
Doc No: 102517247