23 Jul Porcine arterial vacuum lyophilization acellular matrix, as well as preparation method and application thereof
Abstract
The invention provides a porcine arterial vacuum lyophilization acellular matrix, as well as a preparation method and application thereof. The preparation method of the porcine arterial vacuum freeze-drying acellular matrix comprises the following steps: performing vacuum lyophilization to the porcine arterial blood vessels to obtain the best pore by adopting an optimal pre-freezing velocity; rehydrating the matrix with low-concentration detergent and performing acellular treatment; and lyophilizing the matrix and storing at normal temperature. The matrix has the advantages that the porcine aorta is used as a studying object, and a lyophilized acellular blood vessel with proper porosity, mechanical performance and immunogenicity is produced by virtue of optimized preparation conditions; animal in-vivo experiments prove the practicality of three-dimensional blood vessel clinical vascular transplantation, and experimental basis is provided to further clinical application; the preparation method has extremely low cost, so that precious medical resources reach billions of RMB can be saved assuredly; and experimental basis and guidance are provided to clinical application of tissue engineering arterial blood vessels and optimizing research and development of acellular blood vessels.
Year: 2012
Country: CN
Doc No: 102600508