METHOD FOR PREPARING POROUS SCAFFOLD FOR TISSUE ENGINEERING, CELL CULTURE AND CELL DELIVERY

METHOD FOR PREPARING POROUS SCAFFOLD FOR TISSUE ENGINEERING, CELL CULTURE AND CELL DELIVERY

Abstract

27METHOD FOR PREPARING POROUS SCAFFOLD FOR TISSUE ENGINEERING, CELL CULTURE AND CELL DELIVERYAbstractThe present invention relates to a method for preparing a porous scaffold for tissue engineering. It is another object of the present invention to provide a porous scaffold obtainable by the method as above described, and its use for tissue engineering, cell culture and cell delivery. The method of the invention10 comprises the steps consisting of: a) preparing an alkaline aqueous solution comprising an amount of at least one polysaccharide, an amount of a cross-linking agent and an amount of a porogen agent b) transforming the solution into a hydrogel by placing said solution at a temperature from about 4°C to about 80°C for a sufficient time to allow the cross-linking of said amount of15 polysaccharide and c) submerging said hydrogel into an aqueous solution d) washing the porous scaffold obtained at step c).(no suitable figure)

Year:  2012

Country:  SG

Doc No:  185279

 

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