23 Jul Method for culturing cattleya hybrida tissues
Abstract
The invention relates to a method for culturing cattleya hybrida tissues, belonging to the technical field of plant cell engineering. According to the method, quick expanding propagation of the cattleya hybrida tissue culture seedling is realized through callus induction, secondary culture and rooting culture by taking cattleya hybrid shoot as a material. According to the method, the culture medium number is increased, the phenomenon of browning of explants of the conventional culture method of the cattleya hybrida stem tip is effectively solved, the proliferation coefficient is increased, the highest induction survival rate is realized, the formula of the culture medium is improved on the basis of the MS (Murashige and Skoog) culture medium and KC (Knudson C) culture medium, and the culture medium is easy to prepare and low in cost. The method is a beneficial improvement of the prior art and can provide technical support for industrial development and application of the cattleya hybrida.
Year: 2012
Country: CN
Doc No: 102835311