23 Jul Method for culturing sciatic nerve Schwann cells
Abstract
The invention belongs to the field of cell biology, and relates to a method for culturing sciatic nerve Schwann cells of adult mouse. The invention also comprises a method for acquiring Schwann cells with high purity through a purification process. The obtained Schwann cells with purity reaching 91% are purified to reach a purity of 98%. Results show that in vitro predegeneration promotes proliferation of the Schwann cells and inhibits fibroblast proliferation, so as to obtain Schwann cells with high purity. The method provided by the invention employs steps of culture and purification of in vitro predegeneration sciatic nerve to simply and effectively obtain a large amount of Schwann cells with high purity, so as to provide abundant high-quality Schwann cells for restoration of injured nerve, and the Schwann cells can be used as seed cells in tissue engineering.
Year: 2013
Country: CN
Doc No: 102899290