23 Jul Annulus fibrosus and nucleus pulposus integrated composite biphasic scaffold and construction method thereof
Abstract
The invention discloses an annulus fibrosus and nucleus pulposus integrated composite biphasic scaffold and a construction method thereof, and belongs to the technology of biological tissue engineering. Porcine cancellous bone is used for preparing the outer layer which is a cell-free deminerized bone matrix gelatin ring, pigtail intervertebral disc nucleus gelatinosus tissue is used for preparing the core which is a cell-free nucleus pulposus matrix; and the two materials are frozen and lyophilized, sequentially subjected to ultraviolet irradiation crosslink and chemical reagent EDAC (carbodiimide) and NHS (n-hydroxysuccinimide) crosslink, and compounded. The annulus fibrosus and nucleus pulposus integrated composite biphasic scaffold with the design is good in biocompatibility to avoid immunological rejection reactions, has a composition structure and mechanical performance similar to a human intervertebral disc extracellular matrix, is wide in material source, low in cost and simple in preparation process, can be used for constructing tissue engineering intervertebral disc and repairing intervertebral disc degeneration, and has an excellent clinical application prospect.
Year: 2013
Country: CN
Doc No: 103007351