23 Jul Tissue culture method for improving in-vitro regeneration efficiency of common head cabbage
Abstract
The invention discloses a tissue culture method for improving the in-vitro regeneration efficiency of a common head cabbage. The tissue culture method comprises the steps of at first, taking explant with high regeneration and induction rate; sterilizing by using sodium hypochlorite; carrying out dark culture on a tender leaf on an induction culture medium for 16-20 hours, and then carrying out light culture; directly carrying out the light culture on a tender stem section with an axillary bud on the induction culture medium; transferring induced bud or cluster buds on an enrichment culture medium for enrichment culture; taking a strong single bud for rooting culture; removing a sealing film at room temperature for plantlet hardening after the strong single bud is rooted; washing off the culture medium from the root; and transplanting the bud in a prepared hole plate. With the adoption of the tissue culture method provided by the invention, the problem that explant sterilization time is different can be solved, and the problem that the regeneration coefficient in an in-vitro regeneration and induction process of the common head cabbage is low can be solved. With the adoption of the tissue culture method provided by the invention, the most efficient regeneration system of the common head cabbage can be established on the aspects of the sterilization time, an explant taking part, and different hormone combinations, and lays the foundation for the storage of genetic resources of the common head cabbage and the development of breeding of common head cabbage genetic engineering.
Year: 2013
Country: CN
Doc No: 103125398