23 Jul Method for inducing cloned cells of large-scale experimental animal into pluripotent stem cells
Abstract
The invention relates to a method for obtaining pluripotent stem cells with high propagation capacity and high differentiation potential of a large-scale experimental animal by using cloned cells of the large-scale experimental animal as target cells to perform induction of the pluripotent stem cells, optimization of in vitro culture conditions and functional verification, to the field of interdiscipline of animal resource science and cell biology. The pluripotent stem cells cultured by the method is pollution-free, high in cell purity and stable in cell quality and passage growth, so that that pluripotent stem cells are suitable for large-scale culture. The pluripotent stem cells of the large-scale experimental animal can be used for effectively performing lasting and continuous study on the same object of study, provide an object of study with constant characteristics for life science study, can be used as standard cells in the study fields of cell engineering, tissue engineering and the like, and can be used in a plurality of fields of the life science study, so that the pluripotent stem cells have far-reaching significance for the life science study in China.
Year: 2013
Country: CN
Doc No: 103160540