23 Jul Reagent for acellular processing of animal skin tissue, and processing method thereof
Abstract
The invention relates to the technical field of acellular processing of an animal skin tissue in biomedical engineering, and particularly relates to a reagent for acellular processing of a animal skin tissue, and a processing method thereof. The reagent is a solution prepared from SDS (sodium dodecyl sulfonate), NaOH and water. The processing method comprises the following steps of: A, acellular processing of SDS.NaOH; B, glutaraldehyde cross-linking; and C, sterilization. In the technical scheme disclosed by the invention, the animal skin tissue is directly processed by adopting the solution containing the SDS and the NaOH to obtain an acellular dermal matrix. Compared with other methods, the processing method provided by the invention has the characteristics of being simple and convenient, efficient, cheap, suitable for popularization and the like. The dermal matrix processed by the method is thorough in cell removal, a basement membrane complex is completely reserved, and the contents of collagen IV, fibronectin, elastin, HLA-DR (human leukocyte antigen DR) and the like in derma are very low.
Year: 2013
Country: CN
Doc No: 103436489