23 Jul Gene transfer method with corn ovule as receptor
Abstract
The invention relates to a gene transfer method with corn ovule as a receptor. The invention belongs to the technical field of plant genetic engineering. With an agrobacterium-infection-mediated genetic transformation principle, pollinated corn ovules are adopted as receptors; the ovule walls are pieced at placed close to micropyles; agrobacterium infection liquid comprising target gene is dropped into the ovules; through steps such as co-culture, immature embryo induction, differentiation and regeneration, rooting, molecular identification, and the like, transgenic corn plants are finally obtained. With the method, technical difficulties such as limited genotypes, complicated tissue culture operation, long transferring period, and the like of traditional corn transgenic methods can be effectively solved. Also, with the method, transgenic plant fertility is improved, target gene expression efficiency is high, and inheritance is stable. The method has great significance upon corn genetic engineering theoretical research and genetic breeding practices.
Year: 2014
Country: CN
Doc No: 103497970