23 Jul Separation and purification method of goat mammary epithelial cells
Abstract
The invention relates to the field of tissue and cell engineering and particularly provides a separation and purification method of goat mammary epithelial cells. The method mainly comprises the following steps: 1) tissue sampling: collecting a goat mammary acinus tissue in a sterile manner; 2) cell separation: separating the mammary epithelial cells and fibroblasts by using a tissue block culture method; 3) cell purification: removing the fibroblasts by combining a differential digestion method with a differential adhesion method to obtain the purified mammary epithelial cells. The method provided by the invention is simple and convenient to operate, and the mammary epithelial cells which are fully purified can be obtained through the conventional cell culture operation. The mammary epithelial cells obtained by adopting the method provided by the invention have the advantages of tidy shapes and exuberant proliferation, and can be applied to research of breast growth and development, lactation mechanism and verification of effectiveness of a specific expression carrier of the mammary tissue.
Year: 2014
Country: CN
Doc No: 103525752