23 Jul Application of ALDH2 (Alcohol Dehydrogenase 2) gene in detection of upper tract urothelial carcinomas
Abstract
The invention discloses application of an ALDH2 (Alcohol Dehydrogenase 2) gene in detection of upper tract urothelial carcinomas (UTUC), and belongs to the technical field of genetic engineering. The application is as follows: a comparison is performed on the expression quantity of mRNA (Messenger Ribonucleic Acid) of the ALDH2 gene of an urothelium tissue sample and the expression quantity of mRNA in the ALDH2 gene of a normal urothelium tissue sample through RT-PCR (Reverse Transcription-Polymerase Chain Reaction) reaction; if the expression quantity of mRNA in the ALDH2 gene of the sample is less than that of mRNA in the normal ALDH2 gene, the sample can be determined as an upper tract urothelial carcinomas sample; the sample urothelium tissue also can be subjected to immunohistochemistry detection, if the staining index of the sample urothelium tissue is not greater than 4, the ALDH2 of the sample urothelium tissue is of high expression, and such sample can be determined as the upper tract urothelial carcinomas sample. The application has the advantages that a novel diagnostic index is provided for the diagnosis of an UTUC patient, and the survival difference between T2 and T3 patients can be forecasted through the ALDH2 gene.
Year: 2014
Country: CN
Doc No: 103558383