23 Jul Tobacco squalene synthase?protein, tobacco squalene synthase?gene and application
Abstract
The invention discloses a tobacco squalene synthase?protein, the?amino acid sequence?of which is as shown in SEQIDNO: 1. Tobacco squalene synthase?gene (i) NtSQS (/i) is a?key regulatory gene?in tobacco sterol synthesis pathway?and has a specific high expression level in?tender?tissue.?Virus mediated?gene silencing?vector TRV-NtSQS which is constructed is?inoculated to?Ben’s tobacco?so as to obtain a plant with high efficiency of silence. By detection of?phytosterol?content in?Ben’s tobacco, the phytosterol?content in the?gene silencing?plant is found to be?observably?decreased?by 41.26%. The above?phenomenon?indicates that?the sterol content?in a plant can be significantly reduced by silencing?the gene. Tobacco squalene synthase?gene (i) NtSQS (/i) is the?key regulatory genein tobacco sterol synthesis pathway, can be used to adjust the sterol content?in tobacco and contributes to?genetic engineering breeding?of low sterol?high quality tobacco.
Year: 2014
Country: CN
Doc No: 103820424