23 Jul Small-caliber in-situ tissue engineering blood vessel and construction method thereof
Abstract
The invention discloses small-caliber in-situ tissue engineering blood vessel and a construction method thereof. The method comprises the following steps: (1) adding pyridine and nitrophenyl chloroformate into poly caprolactone methylene chloride solution for reaction, precipitation and drying; (2) adding polyethylene glycol diamine methylene chloride solution into the precipitate methylene chloride solution obtained in the step (1) for reaction, precipitation and drying to obtain poly caprolactone polyethyleneglycol diamine periodic copolymer; (3) adopting the electrospinning technology to prepare a tube type support material taking the mixed solution of the poly caprolactone polyethyleneglycol diamine periodic copolymer and poly trimethylene carbonic ester; (4) immersing the tube type support material into heparin solution containing EDC/NHS to conduct heparinized modification to obtain the small-caliber in-situ tissue engineering blood vessel. The in-situ tissue engineering blood vessel is provided with mechanical property similar to natural vessel and favorable blood compatibility, allows ingrowth from somatic sells while degrading in a body gradually; a finished product is easy to preserve.
Year: 2014
Country: CN
Doc No: 103961750